2009-2012 PhD, Biochemistry, University of Vermont, Burlington, VT
Thesis: The role of flow in regulating the prothrombinase complex

2005-2006 Graduate Studies, Physical Chemistry, University of Colorado, Boulder, CO

2001-2005 AB, Chemistry, Smith College, Northampton, MA
Thesis: Calculations of methyl hydroperoxide overtone spectra

2026-present Assistant Research Scientist, Life Sciences Institute, University of Michigan

2022-2026 Research Investigator, Life Sciences Institute, University of Michigan

2017-2022 Postdoctoral Research Fellow, Life Sciences Institute, University of Michigan

2012-2017 Postdoctoral Fellow/Associate, Department of Biochemistry, University of Vermont

2007-2009 Research Technician, Department of Biochemistry, University of Vermont

2004-2005 Student Fellow, Kahn Liberal Arts Institute, Smith College

2026-2028 American Society of Hematology Junior Faculty Scholar Award ($150,000)

2021-2020 University of Michigan Frankel Cardiovascular Center Impact Research Ignitor Grant ($90,076)

2018-2020 National Bleeding Disorders Foundation Judith Graham Pool Postdoctoral Fellowship ($104,000)

2026 Junior Faculty Scholar Award, American Society of Hematology

2025 Marilyn Kroin Beck Mentorship Award, Life Sciences Institute, University of Michigan

2025 Early Career Travel Award, International Society of Thrombosis and Haemostasis

2017 Young Investigator Travel Award, International Society of Thrombosis and Haemostasis

2013 Early Career Award, 8th Annual Platelet Colloquium

2011 U.S. New Investigator Travel Award, International Society of Thrombosis and Haemostasis

2024 Speaker, Thrombosis and Hemostasis Summit of North America

2024 Moderator, Thrombosis and Hemostasis Summit of North America

2021 Speaker, Plasminogen Activation and Extracellular Proteolysis Gordon Research Conference

2020 Speaker, International Society of Thrombosis and Haemostasis Virtual Congress

2019 Speaker, Nationak Hemophilia Foundation Bleeding Disorders Conference

2018 Moderator, Plasminogen Activation and Extracellular Proteolysis Gordon Research Conference

2017 Speaker, International Society of Thrombosis and Haemostasis Congress

2016 Moderator, Hemostasis Gordon Research Conference

2013 Speaker, 8th Annual Platelet Colloquium

2026 Journal Review, FEBS Journal

2026 Journal Review, Journal of the Royal Society Interface

2026 Abstract Review, American Heart Association Vascular Discover Conference 

2025 Grant Review, American Heart Association 

2025 Journal Review, Hematologica

2024-2028 Member, ATVB Kenneth M. Brinkhous, Young Investigator Prize in Thrombosis Award Selection
Committee, American Heart Association

2023 Journal Review, Journal of Theoretical Biology

2021 Journal Review, Scientific Reports

2020 Speaker, Ann Arbor Nerd Nite

2020 Journal Review, Journal of Thrombosis and Haemostasis 

2018-2020 Member, Life Sciences Institute Postdoctoral Committee, University of Michigan

2016 Journal Review, Blood Reviews

2015-2022 Judge, Vermont State Science Fair

2014 Teaching Assistant, Girls Science Discovery Day, University of Vermont

2012 Member, LCME Institutional Setting Subcommittee, University of Vermont, Larner College of Medicine

• American Heart Association

• American Society of Hematology

• International Society of Thrombosis and Haemostasis

1. Baid S, Holding ML, Laurin T*, Siemieniak D, Ginsburg D, and Haynes LM. (2026) Mapping the mutational landscape for streptokinase binding to plasminogen. bioRxiv. *Undergraduate researcher

2. Holding ML, Pontius MHH, Ward MS*, Akhtar J, Crowell HL, Svilar D, Keeler KM, Haynes LM, Ginsburg D, and Shavit J. (2026) Deconstructing cardiovascular and coagulation-related traits reveals multi-functional snake venom specificity. Evolution, Epub ahead of print. PMID: 41811938 *Undergraduate researcher

3. Ward MS*, Holding ML, Haynes LM, Margres MJ, Matocq MD, and Ginsburg D. (2025) Tandem duplication of serpin genes yields functional variation and snake venom inhibitors. Mol. Biol. Evol., 42, msaf290. PMID: 41218074 *Undergraduate researcher

4. Haynes LM, Holding ML, Woodard J, Siemieniak D, and Ginsburg D. (2025) Thermodynamics and selection of the plasminogen activator inhibitor-1 latency transition. J. Biol. Chem., 12, 110896. PMID: 41197721

5. Holding ML*, Disharoon D*, Haynes LM*, Chakravarthy Paruchuri B, Pontius MHH, Golden K, Shavit HA, Desch KC, Ginsburg D, Sen Gupta A, Cruz Y, Drabeck DH. (2025) Comparative microfluidic and enzymatic analyses reveal multifaceted snake venom resistance and novel VWF behavior in the opossum Monodelphis domestica. PLoS One, 20, e0332686. PMCID: PMC12448354 *Co-authors contributed equally

6. Haynes LM, Holding ML, DiGiovanni HL*, Siemieniak D, and Ginsburg D. (2025) High-throughput amino acid-level characterization of the interactions of plasminogen activator inhibitor-1 with variably divergent proteases. Protein Sci., 34, e70088. *Undergraduate researcher

7. Haynes LM, Huttinger ZM, Yee A, Kretz AC, Holding ML, Siemieniak DR, Lawrence DA, Ginsburg D. (2022) Deep mutational scanning and massively parallel kinetics of plasminogen activator inhibitor-1 functional stability to probe its latency transition. J. Biol. Chem., 298, 102608. PMCID: 36257408

8. Nelson A, Kelley MA, Haynes LM, Leiderman K. (2021) Mathematical models of fibrin polymerization: Past, present, and future. Curr. Opin. Biomed. Engin., 20, 100350.

9. Huttinger ZM*, Haynes LM*, Yee A, Kretz AC, Holding ML, Siemieniak DR. Lawrence DA, Ginsburg D. (2021) Deep mutational scanning of the plasminogen activator inhibitor-1 functional landscape. Sci. Rep., 11, 18827. PMCID: 34552126 *Co-authors contributed equally

10. Orfeo T, Gissel M, Haynes LM, Pusateri A, Mann KG and Brummel-Ziedins KE. (2018) Hemodilution and endothelial cell regulation of whole blood coagulation. Mil Med., 183 (Suppl_1), 174-182. PMCID: 29635586

11. Haynes LM, Orfeo T, Mann KG, Everse SJ, and Brummel-Ziedins, KE. (2017). Probing the dynamics of clot-bound thrombin at venous shear rates, Biophys. J., 112 (8), 1634-1644. PMCID: 28445754

12. Haynes LM, Bouchard BA, Tracy PB, and Mann KG. (2012) Prothrombin activation by platelet-associated prothrombinase proceeds through the prethrombin-2 pathway via a concerted mechanism. J. Biol. Chem., 287 (46), 38647-38655. PMCID: PMC3493909

13. Haynes LM, Orfeo T, and Mann KG (2012) Rivaroxaban delivery and reversal at a venous flow rate, Arterioscler. Thromb. Vasc. Biol., 32 (12), 2877-2883. PMCID: PMC3629550

14. Haynes LM, Dubief YC, and Mann, KG. (2012) Membrane binding events in the initiation and propagation phases of tissue factor initiated zymogen activation under flow, J. Biol. Chem., 287 (8), 5225-5234. PMCID: PMC3031058

15. Wood JP, Silveira JR, Maille NM, Haynes LM, and Tracy PB. (2011) Prothrombin activation on the activated platelet surface optimizes expression of procoagulant activity. Blood, 17 (5), 1710-1718. PMCID: PMC3056595

16. Haynes LM, Dubief YC, Orfeo T, and Mann KG. (2011) Dilutional control of prothrombin activation at physiologically relevant shear rates. Biophys. J., 100 (3), 765-773. PMCID: PMC3031058

17. Haynes LM, Vogelhuber KM, Pippen JL, and Hsieh S. (2005) Effects of torsion on the O-H overtone spectra and direct overtone photolysis of methyl hydroperoxide. J. Chem. Phys., 123(23), 234306.

18. Homitsky SC, Dragulin SM, Haynes LM, and Hsieh S. (2004) O-H stretch overtone excitation in methyl and ethyl hydroperoxides. J. Phys. Chem. A, 108 (44), 9492-9499.

*Denotes presenting author

1. Holding M*, Ward M, Apte S, Haynes L, Margres MJ, Martin D, Matocq M, Schield D, Tarvin RD, and Ginsburg D. "Comparative genomic and functional proteomic interrogation of mammalian resistance to rattlesnake venoms", Evolution 2026, Cleveland, OH, June 2026 (Oral Presentation)

2. Holding M*, Ward M, Haynes L, Margres MJ, Matocq M, and Ginsburg D. "Duplication of rodent serpin genes as molecular fuel for coevolved resistance to snake venoms", Evolution 2026, Cleveland, OH, June 2026 (Poster Presentation)

3. Baid S*, Haynes L, Holding M, Laurin T, Siemeiniak D, and Ginsburg D. "Streptococcus pathogenicity through high-throughput analysis", Microbe 2026, Washington, DC, June 2026. (Poster Presentation)

4. Holding ML, DiGiovanni HL, Siemieniak D, Ginsburg D, and Haynes LM*. “Functional novelty during the evolution of SERPINE1 and SERPINE2 from a common ancestor", Plasminogen Activation and Extracellular Proteolysis Gordon Research Conference, Ventura, CA, February 2026. (Oral Presentation)

5. Haynes L*, Holding M, Siemieniak D, Lawrence D, and Ginsburg D.  "Identification of a vitronectin-induced signaling network in plasminogen activator inhibitor-1", International Society of Thrombosis and Haemostasis Congress. Washington, DC, June 2025. (Moderated Poster Presentation)

6. DiGiovanni H*, Haynes L, Holding M, and Ginsburg D. "Evolution of plasminogen activator inhibitor 1 and protease nexin-1 divergent thrombin specificities", International Society of Thrombosis and Haemostasis Congress. Washington, DC, June 2025. (Poster Presentation)

7. Baid S*, Haynes L, Holding M, Siemieniak D, and Ginsburg D. "High throughput mapping streptokinase-plasminogen interaction reveals residues critical for binding", International Society of Thrombosis and Haemostasis Congress. Washington, DC, June 2025. (Poster Presentation)

8. Haynes LM*, Holding ML, Siemieniak D, Lawrence D, and Ginsburg D. “Mapping the vitronectin allosteric signaling network in plasminogen activator inhibitor-1”, Mutational Scanning Symposium, Cambridge, MA, May 2024. (Oral Presentation, https://tinyurl.com/2evbwss4)

9. Haynes LM*, Holding ML, Trost E, DiGiovanni H, Siemieniak D, and Ginsburg D. (2023) Engineering plasminogen activator inhibitor-1 variants with novel Protease inhibitor activity. Vascular Discovery 2023, Baltimore, MD, April 2023. (Oral Presentation)

10. Holding ML*, Pontius HH, Haynes LM, Shavit J, and Ginsburg D. “Fluorescent fish shed light on the adaptive evolution of cottonmouth venom”, Joint Meeting of Ichthyologists and Herpetologists 2023, Norfolk, VA, July 2023. (Oral Presentation)

11. Haynes LM*, Holding ML, DiGiovanni, Siemieniak D, and Ginsburg D. “Deep mutational scanning relates inhibitor specificity to the evolutionary divergence of its targets”, Evolution 2023, Albuquerque, NM, June 2023. (Oral Presentation)

12. Holding ML*, Pontius HH, Haynes LM, Shavit J, and Ginsburg D. “Fluorescent fish shed light on reciprocal taxonomic specialization of snake venom procoagulant function”, Evolution 2023, Albuquerque, NM, June 2023. (Oral Presentation)

13. Haynes LM*, Holding ML, Siemieniak D, and Ginsburg D. Fingerprinting PAI-1’s protease specificity through deep mutational scanning, Hemostasis Gordon Research Conference, Waterville Valley, NH, August 2022. (Poster Presentation)

14. Haynes LM*, Holding ML, and Ginsburg D. Fingerprinting serine protease inhibitor (SERPIN) specificity, Mutational Scanning Symposium, Toronto, ON, June 2022 (Oral Presentation, https://tinyurl.com/42sathea)

15. Haynes LM*, Huttinger ZM, and Ginsburg D. Deep mutational scanning and high-throughput kinetics to quantitatively assess plasminogen activator inhibitor-2 variants. International Society of Thrombosis and Haemostasis Virtual Congress, June 2020. (Virtual Poster Presentation)

16. Haynes LM*, Huttinger ZM, and Ginsburg D. “High-throughput quantitative characterization of PAI-1’s mutational landscape”, SERPINS2019, Seville, Spain, September 2019 (Oral Presentation)

17. Haynes LM*, Huttinger ZM, and Ginsburg D. “Characterizing plasminogen activator inhibitor-1 as a scaffold for designer SERPIN development” Synthetic Biology Gordon Research Conference, Waterville Valley, NH, July 2019 (Poster Presentation)

18. Huttinger ZM*, Haynes LM*, Yee A, Siemieniak D, and Ginsburg D. “Identification of stabilizing mutations in PAI-1 using phage display libraries and high-throughput sequencing” Plasminogen Activation and Extracellular Proteolysis Gordon Research Conference, Ventura, CA, February 2018. (Poster Presentation)

19. Haynes LM*, Orfeo T, and Brummel-Ziedins KE. "Clot-bound thrombin characterization of plasma clots in a flow reactor", International Society of Thrombosis and Haemostasis Congress, Berlin, Germany, July 2017. (Oral Presentation)

20. Haynes LM*, Orfeo T, Wamsley CE, Everse SJ, and Brummel-Ziedins KE. "Clot-bound thrombin under venous flow conditions", International Society of Thrombosis and Haemostasis Scientific and Standardization Commitee Meeting, Montpelier, France, May 2016. (Poster Presentation)

21. Haynes LM*, Orfeo T, Mann KG, Everse SJ, and Brummel-Ziedins KE. “The resilience of clot-bound thrombin under venous flow conditions.” Hemostasis Gordon Research Conference, Stowe, Vermont, July 2016. (Poster Presentation)

22. Haynes LM* and Tracy PB. "Membrane cholesterol is a critical regulator of platelet-associated prothrombinase complex assembly",  International Society of Thrombosis and Haemostasis Congress, Toronto, ON, June 2015. (Poster Presentation)

23. Bravo MC*, Orfeo T, Gissel M, Lavoie E, Haynes L, and Mann K. "The modulation of prothrombinase lifetime by rivaroxaban", International Society of Thrombosis and Haemostasis Congress, Toronto, ON, June 2015. (Poster Presentation)

24. Ayombil F*, Haynes LM, and Tracy, PB. "Fragment-2 inhibition of prothrombinase reveals a unique enzyme-substrate interface for the platelet-bound enzyme.",  International Society of Thrombosis and Haemostasis Congress, Toronto, ON, June 2015. (Poster Presentation)

25. Haynes LM* and Tracy PB. “The surface of platelet-derived microparticles attenuates their procoagulant nature via the prothrombinase complex and the function of activated protein C” Hemostasis Gordon Research Seminar, Waterville, New Hampshire, July 2014. (Oral Presentation)

26. Haynes LM* and Tracy PB. “The surface of platelet-derived microparticles attenuates their procoagulant nature via the prothrombinase complex and the function of activated protein C” Hemostasis Gordon Research Conference, Waterville, New Hampshire, July 2014. (Poster Presentation)

27. Haynes LM* and Tracy, PB. “Platelet-derived microparticles and prothrombin processing” FASEB Scientific Research Conference: Proteases in Hemostasis and Vascular Biology, Nassau, Bahamas, June 2013. (Poster Presentation)

28. Haynes LM* and Tracy, PB. "Platelet-derived microparticles attenuate the procoagulant nature of platelet-associated prothrombinase",   International Society of Thrombosis and Haemostasis Congress, Toronto, ON, June 2013. (Poster Presentation)

29. Haynes LM*, Bouchard BA, Tracy PB, and Mann, KG. “Platelet prothrombinase activates prothrombin through the prethrombin-2 Pathway via a processive mechanism” Hemostasis Gordon Research Conference, Waterville, New Hampshire, July 2012. (Poster Presentation)

30. Haynes LM*, Dubief YC, and Mann, KG. "The presentations of factor Xa and thrombin under flow", International Society of Thrombosis and Haemostasis Congress, Kyoto, Japan, June 2011. (Poster Presentation)

31. Haynes LM*, Dubief YC, Orfeo T, and Mann, KG. "The effects of flow on the activation of bovine prothrombin by prothrombinase at physiologically relevant shear rates",  Experimental Biology, Anaheim, CA, April 2010. (Poster Presentation)

32. Haynes LM*, Dubief YC, Orfeo T, and Mann, KG. “Dynamics of prothrombin activation under flow” Hemostasis Gordon Research Conference, Waterville, New Hampshire, July 2010. (Poster Presentation)

33. Haynes LM*, Vogelhuber KM*, and Hsieh, S. “Vibrational overtone excitation of methyl hydroperoxide and hydroxyl radical formation” Ohio State University International Symposium on Molecular Spectroscopy, Columbus, Ohio, June 2005. (Oral Presentation)

34. Haynes LM* and Hsieh S. "O-H stretch overtone excitation cross sections for methyl hydroperoxide", American Chemical Society Meeting, August 2024.  (Poster Presentation)