Education
2009-2012 PhD in Biochemistry, University of Vermont, Burlington, Vermont
2005-2006 Graduate Studies in Physical Chemistry, University of Colorado, Boulder, Colorado
2001-2005 BA in Chemistry, cum laude with Highest Honors in Chemistry, Smith College, Northampton, Massachusetts
Research Experience
2022- present Research Investigator, University of Michigan, Life Sciences Institute
2017-2022 Postdoctoral Research Fellow in the laboratory of David Ginsburg at the University of Michigan, Life Sciences Institute
2015-2017 Postdoctoral Associate in the laboratory of Kathleen Brummel-Ziedins at the University of Vermont, Department of Biochemistry
2012-2015 Postdoctoral Fellow in the laboratory of Paula Tracy at the University of Vermont, Department of Biochemistry
2009-2012 Graduate Student in the laboratory of Kenneth Mann at the University of Vermont, Department of Biochemistry
· Dissertation: “The role of flow in regulating the prothrombinase complex”
· Co-mentor: Yves Dubief, Mechanical Engineering
2007-2009 Research Technician in the laboratory of Kenneth Mann at the University of Vermont, Department of Biochemistry
2004-2005 Student Fellow, Smith College, Kahn Liberal Arts Institute
2002-2005 Undergraduate Research Assistant in the laboratory of Shizuka Hsieh, Smith College, Department of Chemistry
· Honor’s Thesis: “Calculations of methyl hydroperoxide overtone spectra”
Teaching Experience
2016-2017 Lecturer, Summer Medical School Prep Program, University of Vermont, Larner College of Medicine (Undergraduate)
2016-2017 Small Group Leader, MD 556: Nutrition, Metabolism, Gastrointestinal Intestinal System in Health & Disease, University of Vermont, Larner College of
Medicine (Medical)
2014-2016 Small Group Leader, BIOC 212: Biochemistry of Health and Human Disease, University of Vermont, Department of Biochemistry (Undergraduate)
2013-2014 Postdoctoral Teaching Assistant and Guest Lecturer, BIOC 302: Introduction to Biochemistry II, University of Vermont, Department of Biochemistry (Graduate)
2012-2013 Postdoctoral Teaching Assistant and Guest Lecturer, BIOC 205: Biochemistry, University of Vermont, Department of Biochemistry (Undergraduate)
2011 Graduate Teaching Assistant, BIOC 302: Introduction to Biochemistry II, University of Vermont, Department of Biochemistry (Graduate)
2010 Graduate Teaching Assistant, BIOC 301: Introduction to Biochemistry I, University of Vermont, Department of Biochemistry (Graduate)
2010 Graduate Teaching Assistant, BIOC 212: Biochemistry of Health and Human Disease, University of Vermont, Department of Biochemistry
(Undergraduate)
Mentoring
I have mentored nine undergraduate/post-baccalaureate students during my graduate and postdoctoral career, as well as four high school students, in addition to co-mentoring two graduate students, a postdoctoral fellow, and a clinical research fellow. Mentoring activities have ranged from supervising work-study students, developing independent research projects for students, training students in the laboratory, and assisting students with graduate and medical school applications.
Funding
2020-2021 COVID-19: Cardiovascular Research Ignitor Grant from the University of Michigan Cardiovascular Center
2018-2020 National Hemophilia Foundation Judith Graham Pool Postdoctoral Fellowship
2012-2015 Postdoctoral Fellow, NIH Thrombosis and Hemostasis Training Grant, University of Vermont
2009-2012 Predoctoral Fellow, NIH Thrombosis and Hemostasis Training Grant, University of Vermont
2005-2006 NSF IGERT Optical Science and Engineering Fellowship, University of Colorado
Invited Talks
2024 Thrombosis and Hemostasis Summit of North America, Chicago, IL
2024 Plasminogen Activation and Extracellular Proteolysis Gordon Research Conference, Ventura, CA
2021 Department of Biomechanical Engineering, Oregon Health Sciences University, Portland, OR
2020 Biorheology Scientific and Standardization Subcommittee of the International Society of Thrombosis and Haemostasis, Virtual Conference
2019 National Hemophilia Foundation Bleeding Disorders Conference, Anaheim, California
2017 Biorheology Scientific and Standardization Subcommittee of the International Society of Thrombosis and Haemostasis, Berlin, Germany
2013 8th Annual Platelet Colloquium, Washington, DC
Honors and Awards
2025 Early Career Travel Award, International Society of Thrombosis and Haemostasis
2017 Elected to the Early Career Advisory Committee, Cardiovascular Research Institute of Vermont, University of Vermont
2017 Young Investigator Travel Award, International Society of Thrombosis and Haemostasis Congress, Berlin, Germany
2013 Early Career Award, 8th Annual Platelet Colloquium
2011 U.S. New Investigator Travel Award, International Society of Thrombosis and Hemostasis Congress, Kyoto, Japan
2005 C. Pauline Burt Prize for “high potential for further study in science”, Smith College, Department of Chemistry
2005 Phi Beta Kappa, Smith College
2005 Sigma Xi, Smith College
2004 American Chemical Society Analytic Chemistry Award, Smith College
Service
2025 Member (ad hoc), American Heart Association Transformational Project Award Vascular Basic Sciences Review Committee
2024-present Member, ATVB Kenneth M. Brinkhous, Young Investigator Prize in Thrombosis Award Selection Committee, American Heart Association
2022-2023 Mentor, Aspirnaut High School Research Program, Life Sciences Institute, University of Michigan
2022 Mentor, Undergraduate Research Opportunity Program, University of Michigan
2022, 2025 Mentor, Perrigo Undergraduate Research Program, Life Sciences Institute, University of Michigan
2020 Speaker, Ann Arbor Nerd Nite (video: https://tinyurl.com/3h3ymkvx)
2018-2020 Member, Life Sciences Institute Postdoctoral Committee, University of Michigan
2018 Discussion Leader, Plasminogen Activation and Extracellular Proteolysis Gordon Research Conference, Ventura, California
2015, 2017, 2022 Judge, Vermont State Science Fair
2016 Discussion Leader, Hemostasis Gordon Research Conference, Stowe, Vermont
2014 Teaching Assistant, Girl’s Science Discovery Day, University of Vermont, Larner College of Medicine
2012 Member, LCME Institutional Setting Subcommittee, University of Vermont, Larner College of Medicine
Reviewer: Haematologia, Journal of Theoretical Biology, Scientific Reports, Blood, Journal of Thrombosis and Hemostasis, Blood Reviews, Molecules
Member: American Heart Association, American Society of Hematology
Professional Development
2020 Workshop Participant, Making Active Learning Work in Hybrid and Online Courses, Center for Research on Learning and Teaching, University of
Michigan
2020 Student, Postdoctoral Short Course in STEM Teaching, Center for Research on Learning and Teaching, University of Michigan
2019 Participant, STEM Communication Fundamentals Workshop, University of Michigan
2019 Workshop Participant, Mentoring Undergraduate and Graduate Student Research, Center for Research on Learning and Teaching, University of
Michigan
2017 Workshop Participant, Teaching Academy Snow Season Retreat, Larner College of Medicine, University of Vermont
2014 Seminar Participant, Designing Group Work that Really Works, Larner College of Medicine, University of Vermont
2014 Seminar Participant, Getting Beyond Covering Content, Larner College of Medicine, University of Vermont
2013 Seminar Participant, Writing Winning Grant Proposals, Larner College of Medicine, University of Vermont
Publications
1. Haynes LM, Holding ML, DiGiovanni HL*, Siemieniak D, and Ginsburg D. (2025) High-throughput amino acid-level characterization of the interactions of plasminogen activator inhibitor-1 with variably divergent proteases. Protein Sci., 34, e70088.
*Undergraduate researcher
2. Haynes LM, Huttinger ZM, Yee A, Kretz AC, Holding ML, Siemieniak DR,. Lawrence DA, Ginsburg D. (2022) Deep mutational scanning and massively parallel kinetics of plasminogen activator inhibitor-1 functional stability to probe its latency transition. J. Biol. Chem., 298, 102608. PMCID: 36257408
3. Nelson A, Kelley MA, Haynes LM, Leiderman K. (2021) Mathematical models of fibrin polymerization: Past, present, and future. Curr. Opin. Biomed. Engin., 20, 100350.
4. Huttinger ZM*, Haynes LM*, Yee A, Kretz AC, Holding ML, Siemieniak DR,. Lawrence DA, Ginsburg D. (2021) Deep mutational scanning of the plasminogen activator inhibitor-1 functional landscape. Sci. Rep., 11, 18827. PMCID: 34552126 *Co-authors contributed equally
5. Orfeo T, Gissel M, Haynes LM, Pusateri A, Mann KG and Brummel-Ziedins KE. (2018) Hemodilution and endothelial cell regulation of whole blood coagulation. Mil Med., 183 (Suppl_1), 174-182. PMCID: 29635586
6. Haynes LM, Orfeo T, Mann KG, Everse SJ, and Brummel-Ziedins, KE. (2017). Probing the dynamics of clot-bound thrombin at venous shear rates, Biophys. J., 112 (8), 1634-1644. PMCID: 28445754
7. Haynes LM, Bouchard BA, Tracy PB, and Mann KG. (2012) Prothrombin activation by platelet-associated prothrombinase proceeds through the prethrombin-2 pathway via a concerted mechanism. J. Biol. Chem., 287 (46), 38647-38655. PMCID: PMC3493909
8. Haynes LM, Orfeo T, and Mann KG (2012) Rivaroxaban delivery and reversal at a venous flow rate, Arterioscler. Thromb. Vasc. Biol., 32 (12), 2877-2883. PMCID: PMC3629550
9. Haynes LM, Dubief YC, and Mann, KG. (2012) Membrane binding events in the initiation and propagation phases of tissue factor initiated zymogen activation under flow, J. Biol. Chem., 287 (8), 5225-5234. PMCID: PMC3031058
10. Wood JP, Silveira JR, Maille NM, Haynes LM, and Tracy PB. (2011) Prothrombin activation on the activated platelet surface optimizes expression of procoagulant activity. Blood, 17 (5), 1710-1718. PMCID: PMC3056595
11. Haynes LM, Dubief YC, Orfeo T, and Mann KG. (2011) Dilutional control of prothrombin activation at physiologically relevant shear rates. Biophys. J., 100 (3), 765-773. PMCID: PMC3031058
12. Haynes LM, Vogelhuber KM, Pippen JL, and Hsieh S. (2005) Effects of torsion on the O-H overtone spectra and direct overtone photolysis of methyl hydroperoxide. J. Chem. Phys., 123(23), 234306.
13. Homitsky SC, Dragulin SM, Haynes LM, and Hsieh S. (2004) O-H stretch overtone excitation in methyl and ethyl hydroperoxides. J. Phys. Chem. A, 108 (44), 9492-9499.
Book Chapter
1. Mann KG, Haynes LM, and Brummel-Ziedins KE. “Normal Coagulation.” In Anton NS, Sidway, Perler BA, eds. Rutherford’s Vascular Surgery and Endovascular Therapy 9th ed. Philadelphia: Elsevier Health Sciences, 2018. p. 448-64
Preprints
1. Holding M*, Disharoon D*, Haynes L*, Paruchuri BC, Pontius MHH, Golden K, Shavit JA, Desch K, Ginsburg D, Sen Gupta A, Cruz Y, and Drabeck DH. (2025) Characterizing venom resistance in Monodelphis domestica yields new insights into mammalian blood physiology. bioRxiv [preprint].
*Co-authors contributed equally
2. Ward MS*, Holding ML, Haynes LM, and Ginsburg D. (2025) Tandem duplication of serpin genes yields functional variation and snake venom inhibitors. bioRxiv [preprint].
*Undergraduate researcher
Published Abstracts
1. Haynes LM, Huttinger ZM, and Ginsburg D. (2020) Deep mutational scanning and high-throughput kinetics to quantitatively assess plasminogen activator inhibitor-2 variants. Res Pract Thromb Haemost 4 (S1). (Virtual Poster Presentation)
2. Haynes LM, Orfeo T, and Brummel-Ziedins KE. (2017) Clot-bound thrombin characterization of plasma clots in a flow reactor. Res. Prac. Thromb. Haemost. 1 (S1), 107. (Oral Presentation)
3. Haynes LM, Orfeo T, Wamsley CE, Everse SJ, and Brummel-Ziedins KE. (2016) Clot-bound thrombin under venous flow conditions. J. Thromb. Haemost. 14 (S1), 7. (Poster Presentation)
4. Haynes LM and Tracy PB. (2015) Membrane cholesterol is a critical regulator of platelet-associated prothrombinase complex assembly. J. Thromb. Haemost. 13 (S2), 313. (Poster Presentation)
5. Bravo MC, Orfeo T, Gissel M, Lavoie E, Haynes L. (2015) and Mann K. The modulation of prothrombinase lifetime by rivaroxaban. J. Thromb. Haemost. 13 (S2), 397. (Poster Presentation)
6. Ayombil F, Haynes LM, and Tracy, PB (2015) Fragment-2 inhibition of prothrombinase reveals a unique enzyme-substrate interface for the platelet-bound enzyme. J. Thromb. Haemost. 13 (S2), 557. (Poster Presentation)
7. Haynes LM and Tracy, PB. (2013) Platelet-derived microparticles attenuate the procoagulant nature of platelet-associate prothrombinase. J. Thromb. Haemost. 11 (S2), 423. (Poster Presentation)
8. Haynes LM, Dubief YC, and Mann, KG. (2011) The presentations of factor Xa and thrombin under flow. J. Thromb. Haemost. 9 (S2), 346. (Poster Presentation)
9. Haynes LM, Dubief YC, Orfeo T, and Mann, KG. (2010). The effects of flow on the activation of bovine prothrombin by prothrombinase at physiologically relevant shear rates. FASEB J. 24, 835.4. (Poster Presentation)
10. Haynes LM and Hsieh S. (2004) O-H stretch overtone excitation cross sections for methyl hydroperoxide. Abstr. Pap. Am. Chem. Soc., 228(2), U275. (Poster Presentation)
Unpublished Abstracts
1. Haynes LM, Holding ML, Siemieniak D, Lawrence D, and Ginsburg D. “Mapping the vitronectin allosteric signaling network in plasminogen activator inhibitor-1”, Mutational Scanning Symposium, Cambridge, MA, May 2024. (Oral Presentation, https://tinyurl.com/2evbwss4)
2. Baid S, Haynes LM, Holding ML, and Ginsburg D. “Analysis of streptokinase interaction with plasminogen by deep mutational scanning”, Mutational Scanning Symposium, Cambridge, MA, May 2024. (Poster Presentation)
3. Holding ML, Pontius HH, Haynes LM, Shavit J, and Ginsburg D. “Fluorescent fish shed light on the adaptive evolution of cottonmouth venom”, Joint Meeting of Ichthyologists and Herpetologists 2023, Norfolk, VA, July, 2023. (Oral Presentation)
4. Haynes LM, Holding ML, DiGiovanni, Siemieniak D, and Ginsburg D. “Deep mutational scanning relates inhibitor specificity to the evolutionary divergence of its targets”, Evolution 2023, Albuquerque, NM, June 2023. (Oral Presentation)
5. Holding ML, Pontius HH, Haynes LM, Shavit J, and Ginsburg D. “Fluorescent fish shed light on reciprocal taxonomic specialization of snake venom procoagulant function”, Evolution 2023, Albuquerque, NM, June 2023. (Oral Presentation)
6. Haynes LM, Holding ML, Trost E, DiGiovanni H, Siemieniak D, and Ginsburg D. “Engineering Plasminogen Activator Inhibitor-1 Variants with Novel Protease Inhibitor Activity”, Vascular Discover, Boston, MA, May 2023. (Oral Presentation)
7. Haynes LM, Holding ML, Siemieniak D, and Ginsburg D. Fingerprinting PAI-1’s protease specificity through deep mutational scanning, Hemostasis Gordon Research Conference, Waterville Valley, NH, August 2022. (Poster Presentation)
8. Haynes LM, Holding ML, and Ginsburg D. Fingerprinting serine protease inhibitor (SERPIN) specificity, Mutational Scanning Symposium, Toronto, Ontario, June 2022 (Oral Presentation, https://tinyurl.com/42sathea)
9. Haynes LM, Huttinger ZM, and Ginsburg D. “High-throughput quantitative characterization of PAI-1’s mutational landscape”, SERPINS2019, Seville, Spain, September 2019 (Oral Presentation)
10. Haynes LM, Huttinger ZM, and Ginsburg D. “Characterizing plasminogen activator inhibitor-1 as a scaffold for designer SERPIN development” Synthetic Biology Gordon Research Conference, Waterville Valley, New Hampshire, July 2019 (Poster Presentation)
11. Huttinger ZM, Haynes LM, Yee A, Siemieniak D, and Ginsburg D. “Identification of stabilizing mutations in PAI-1 using phage display libraries and high-throughput sequencing” Plasminogen Activation and Extracellular Proteolysis Gordon Research Conference, Ventura, California, February 2018. (Poster Presentation)
12. Haynes LM and Tracy PB. “Summer medical school prep program:
Giving undergraduate students a taste of medical school”, Teaching Academy Snow Season Retreat, Larner College of Medicine, Burlington, Vermont, January 2017. (Poster Presentation)
13. Haynes LM, Orfeo T, Mann KG, Everse SJ, and Brummel-Ziedins KE. “The resilience of clot-bound thrombin under venous flow conditions.” Hemostasis Gordon Research Conference, Stowe, Vermont, July 2016. (Poster Presentation)
14. Haynes LM and Tracy PB. “The surface of platelet-derived microparticles attenuates their procoagulant nature via the prothrombinase complex and the function of activated protein C” Hemostasis Gordon Research Seminar, Waterville, New Hampshire, July 2014. (Oral Presentation)
15. Haynes LM and Tracy PB. “The surface of platelet-derived microparticles attenuates their procoagulant nature via the prothrombinase complex and the function of activated protein C” Hemostasis Gordon Research Conference, Waterville, New Hampshire, July 2014. (Poster Presentation)
16. Ayombil F, Haynes LM, and Tracy PB. “Unique structural features of platelet-associated prothrombinase prevent inhibition by prothrombin fragment-2” Hemostasis Gordon Research Conference, Waterville, New Hampshire, July 2014. (Poster Presentation)
17. Haynes LM and Tracy, PB. “Platelet-derived microparticles and prothrombin processing” FASEB Scientific Research Conference: Proteases in Hemostasis and Vascular Biology, Nassau, Bahamas, June 2013. (Poster Presentation)
18. Haynes LM, Bouchard BA, Tracy PB, and Mann, KG. “Platelet prothrombinase activates prothrombin through the prethrombin-2 Pathway via a processive mechanism” Hemostasis Gordon Research Conference, Waterville, New Hampshire, July 2012. (Poster Presentation)
19. Haynes LM, Dubief YC, Orfeo T, and Mann, KG. “Dynamics of prothrombin activation under flow” Hemostasis Gordon Research Conference, Waterville, New Hampshire, July 2010. (Poster Presentation)
20. Haynes LM, Vogelhuber KM, and Hsieh, S. “Vibrational overtone excitation of methyl hydroperoxide and hydroxyl radical formation” Ohio State University International Symposium on Molecular Spectroscopy, Columbus, Ohio, June 2005. (Oral Presentation)